Cytation 1 Cell Imaging Multi-Mode Reader
Cytation™ 1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1’s multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.
- Affordable, patented, quantitative digital microscopy and optional multi-mode microplate detection
- Augmented Microscopy™ using Gen5 software for seamless image capture to quantitative publication ready data with no extensive training required
- Fluorescence and high contrast brightfield imaging from 1.25x to 60x to capture large regions of interest or intracellular details
- Affordable automation: automated XY stage, focus, exposure, image capture and LED intensity
- More than 20 imaging filter/LED cube colors reach many applications
- Cell friendly design: 4-Zone incubation to 45°C with Condensation Control, CO2/O2 control, angled injectors and lidded plate handling
- High performance filter-based fluorescence detection with convenience of monochromator based UV-Vis absorbance
- Peltier Cooling Module maintains environmental stability for uncompromised assay integrity
Seahorse XF Imaging and Normalization System
Cytation 1 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. XFe Analyzers simultaneously measure the two major cellular energy-producing pathways, mitochondrial respiration and glycolysis, in live cells and in real time. The integrated solution with Cytation 1 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation.
WHAT’S NEW
Special Imaging Offer! Purchase a Lionheart or Cytation and receive patented Laser Autofocus Free!
APPLICATIONS
With the ability to collect phenotypic and quantitative data in a single experiment, Cytation 1 is a powerful tool for imaging applications, and conventional multi-mode applications.
- Cell migration and invasion assays
- Live cell imaging
- Label-free cell counting
- Multi-mode reader applications
- Immunofluorescence
- Phenotypic assays
The Scratch Assay Starter Kit automates cell migration and wound healing assays
AutoScratch automatically makes consistent, repeatable scratch wounds.
The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).
AutoScratch creates scratch wounds with excellent performance, compared to manual methods.
HeLa cells treated with SmartFlare™ CY5-GAPDH Reagent at 20x
High contrast brightfield for label-free cell counting.
These are just a few examples of the many important applications for Cytation 1. Visit our Applications page to see more.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
FEATURES
Digital microscopy and multi-mode detection
Collect both phenotypic data and quantitative data from one instrument. Image and data analysis are provided with Gen5 software for all applications.
Easy imaging workflow automation
BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.
Fluorescence and high contrast brightfield imaging
The powerful imaging module in Cytation 1 offers four color channels plus brightfield, from 1.25x to 60x to capture large regions of interest or intracellular details. More than 15 filter/LED cubes are available, supporting a broad range of imaging applications.
Cytation 1 has an automated XY stage, autofocus, auto exposure and auto LED intensity for efficient capture of high quality images. The automated features in Cytation 1 and Gen5 make it easy for you to find the region of interest, then quickly find the best settings to capture the images. Gen5 software is simple to use, without extensive training.
More than 20 filter/LED color cubes
BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.
Cell friendly design
Live cell assays require an optimal environment. Cytation 1 has temperature control and shaking, plus CO2 / O2 control, to facilitate longer-term kinetic studies.
High performance and convenience
To optimize multi-mode detection, Cytation 1 has high performance filters for fluorescence and monochromator-based UV-Vis absorbance for convenient wavelength selection in 1 nm increments.
Peltier Cooling for environmental stability
The Peltier Cooling Module promotes a rapid interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.
GENERAL
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | Monochromator: 6- to 384-well plates Filters: 6- to 1536-well plates Imaging: 6- to 1536-well plates |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates |
| Temperature control | 4-Zone incubation to 45 °C with Condensation Control™; Variation +0.2 °C at 37 °C |
| Cooling | Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides rapid cooling after incubated processes. |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Microplate Reader and Imager Software Gen5 Image+ and Image Prime software available for full image analysis Gen5 Secure available for 21 CFR Part 11 |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | 0 – 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller Models for both CO2 and O2 or CO2 only are available |
IMAGING SYSTEM
| Imaging mode | Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x) |
| Imaging method | Single color, multi-color, montage, time lapse, z-stacking |
| Image processing | Z-projection, image stitching |
| Camera | 16-bit gray scale, Sony |
| Objective capacity | 2 user-replaceable objectives |
| Objectives available | 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Automated functions | Autofocus, auto LED intensity, auto exposure |
| Autofocus method | Image-based autofocus User-trained autofocus Laser autofocus (option) |
| Positional controls | Software control |
| Image collection rate | Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutes Laser autofocus: |
| Image Analysis Software option | Gen5 Image+: Image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features |
FLUORESCENCE INTENSITY
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Deep blocking band pass filters / dichroic mirrors |
| Wavelength range | Filters: 200 – 700 nm (850 nm option) |
| Dynamic range | 7 decades |
| Sensitivity | Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate) |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
LUMINESCENCE
| Wavelength range | 300 – 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | 10 amol ATP (flash) 100 amol (glow) |
FLUORESCENCE POLARIZATION
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 400 nm – 700 nm |
| Sensitivity | 1.2 mP standard deviation at 1 nM fluorescein |
TIME-RESOLVED FLUORESCENCE
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Sensitivity | Europium 40 fM (4 amol/well, 384-well plate) |
ABSORBANCE
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | Monochromator |
| Wavelength range | 200 – 999 nm, 1 nm increment |
| Monochromator bandwidth | 2.4 nm |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | + 2 nm |
| Monochromator wavelength repeatability | + 0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
REAGENT INJECTORS (OPTION)
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 – 1000 µL in 1 µL increments |
| Plate geometry | 6- to 384-well microplates |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 – 200 µL |
PHYSICAL CHARACTERISTICS
| Power | 100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min) |
| Dimensions | 20″ D x 16.5″ W x 17.5″ H (50.8 cm x 41.91 cm x 44.5 cm) |
| Weight | 65 lbs (29 Kg) |
REGULATORY
| Regulatory | CE and TUV marked. Models for In Vitro Diagnostic use are available. |
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Fluorescence and high contrast brightfield imaging
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