Lionheart™ FX Automated Microscope
Lionheart™ FX Automated Microscope is a compact, inclusive microscopy system for a broad range of imaging workflows. It offers up to 60x air; 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40°C and effective containment for CO2/O2 control, a humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays. Lionheart FX and Gen5 together comprise Augmented Microscopy™ – the automation of image capture, processing, analysis and development of publication-ready images and data.
- Automated digital microscopy up to 60x air; 60x and 100x oil immersion
- Fluorescence, brightfield, color brightfield and phase contrast imaging
- Label-free cell counting for cell proliferation studies
- Live cell assay support with environmental controls
- More than 20 imaging filter/LED cube colors reach many applications
- Compact design for quick installation and setup
WHAT’S NEW
The Scratch Assay Starter Kit automates cell migration and wound healing assays, used along with BioTek’s Lionheart FX Automated Microscope or Cytation 5 Cell Imaging Multi-Mode Reader.
Special Imaging Offer! Purchase a Lionheart or Cytation and receive patented Laser Autofocus Free!
APPLICATIONS
Lionheart FX is designed for easy implementation of a broad range of fixed and live cell assay workflows. BioTek’s concept of Augmented Microscopy™ brings automated image capture, processing, analysis and publication-ready images and data to the benchtop – for many applications, including these important areas:
- Cell migration and invasion assays
- Live cell imaging
- High resolution microscopy
- Immunofluorescence
- Phenotypic assays
- Histology
The Scratch Assay Starter Kit automates cell migration and wound healing assays:
AutoScratch automatically makes consistent, repeatable scratch wounds.
The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).
AutoScratch creates scratch wounds with excellent performance, compared to manual methods.
Adding 10 µM ATP activates HeLa endogenously expressed P2Y receptor
FEATURES
Automated digital microscopy up to 100x
Lionheart FX with Augmented Microscopy automates make your imaging workflow to yield amazing images and powerful data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.
Four imaging modes, multiple imaging process
With brightfield, color brightfield, phase contrast and fluorescence imaging in four channels – and nearly twenty available filter/LED cubes, Lionheart FX offers fantastic versatility that isn’t limited to image capture. Follow your imaging processes through z-stacking to z-projection, montage collection to stitching and digital phase contrast to analysis, all one hardware and software platform.
Kinetic live cell assay support
Several options are available to support live cell assays with Lionheart FX, including an environmental cover to maintain temperature and gas levels within the system. Long-term live cell kinetics are supported, using the unique humidity chamber that fits on the stage itself, keeping the live cell in a completely nurturing environment. A dual reagent injector provides quick inject/image capability for observing fast reactions.
Quick installation and easy setup
Fifteen minutes is all it takes to install and set up Lionheart FX for image capture and analysis. The filter/LED cubes and objectives are accessed from the front panel, and tool-free connections for injectors, gas control and the environmental cover add to the easy installation. Its small size takes up minimal benchtop space.
GENERAL
| Microplate types | 6- to 1536-well plates |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Support for labware up to 1.5″ tall |
| Temperature control | Incubation to 40 °C with optional environmental control cover |
| Software | Gen5 Microplate Reader and Imager Software included Gen5 Image+ and Image Prime Software available for advanced image analysis (option) Gen5 Secure for 21 CFR Part 11 Compliance (option) |
| CO2 and O2 control (option) | 0 – 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller |
| Environmental control cover | Top cover for light tight imaging and incubation control (option) |
| X/Y Stage resolution | Lead screw driven stage with 0.1 µm resolution |
| Humidity control | Humidity chamber with rapid gas recharge (option) |
IMAGING SYSTEM
| Imaging mode | Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast |
| Imaging method | Single color, multi-color, montage, time lapse, Z-stacking, burst mode |
| Light source | High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
| Camera | 16-bit grayscale Sony CMOS |
| Camera binning | Optional 2×2 binning for focus and/or image capture |
| Camera exposure range | 5 milliseconds to 4 seconds |
| Image outputs available | Raw Images: 16-bit TIFF Saved Images: TIF, JPG, BMP, PNG, EMF, GIF Movies: MP4, WMV |
| Objective capacity | 6 onboard, user-replaceable objectives |
| Objectives available | Fluorescence: Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 Oil: 60x, NA:1.42; 100x, NA: 1.40 Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60 |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 |
| Automated functions | Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
| Autofocus method | Image-based autofocus Laser autofocus option |
| Image collection rate | Single well fastest frame rate capture: *Full Resolution: up to 10 frames per second for single color images *2×2 Binning: up to 20 frames per second for single color images |
| Microscope stage control | Gen5 Software Control Optional joystick controller |
REAGENT INJECTORS (OPTION)
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri and cell culture dishes, chamber slides |
| Dead volume | <1.65 mL with back flush |
| Dispense tip options | Aligned tip – aligned with optical path for dispensing for fast kinetic assays Offset tip – dispensing is offset from the optical path |
| Dispense volume | 5 – 1000 µL in 1 µL increments |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 – 200 µL |
PHYSICAL CHARACTERISTICS
| Power | 250 Watts maximum consumption. |
| Dimensions | With cover closed or without cover: 18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm) With cover fully open: 18.3″ D, 17.9″ W, 27.5″ H (46.5 cm x 45.5 cm x 69.8 cm) |
| Weight | Without environmental control cover: 51 lbs (23.1 kg) With environmental control cover: 58 lbs (26.3 kg) |
REGULATORY
| Regulatory | CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. |
LFX
Lionheart FX Automated Live Cell Imager. Fluorescence, brightfield, color brightfield and phase contrast imaging capabililty. Includes base Gen5 software. Objectives, filter/LED cubes, accessories and software upgrades sold separately.
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