Lionheart LX Automated Microscope
BioTek’s new Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.
- Affordable automated digital microscopy
- High contrast brightfield, color brightfield and fluorescence imaging
- Augmented Microscopy: Easy imaging workflow automation
- Quick analyze: instant cell count and confluence measurement
- More than 20 imaging filter/LED cube colors reach many applications
- Integrated, compact design offers quick installation and setup
APPLICATIONS
- Cell migration and invasion assays
- Cell counting
- Endpoint live cell assays (apoptosis, autophagy, cytotoxicity and others)
- Histology (H&E)
- Immunofluorescence
- Label-free cell counting
- Confluence
High contrast brightfield for label-free cell counting.
FEATURES
Lionheart LX has an automated 6-objective turret and can contain up to 4 LED/filter cubes for multi-channel image capture. The precise automated stage and autofocus, auto exposure and other Gen5 software features enable simple, automated image capture and analysis.
Four imaging modes, multiple imaging processes
With brightfield, color brightfield and fluorescence imaging in four channels – and more than twenty available filter/LED cubes, Lionheart LX offers fantastic versatility that isn’t limited to image capture. Imaging processes from z-stacking to z-projection, montage collection to stitching; plus analysis from label-free cell counting to mitochrondrial membrane potential are all accomplished with Lionheart LX and Gen5 software.
Easy imaging workflow automation
BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.
Instant cell count and confluence measurement
Get cell counts and confluence calculations directly on the live camera feed. Gen5 software controls Lionheart LX to quickly find the region of interest and display counts onscreen without needing to capture the image. The quick analyze function works for samples in a variety of labware.
Quick installation and easy setup
Lionheart LX integrates all microscopy hardware into a very compact footprint, saving valuable bench space. The easily accessible objectives and LED/filter cubes make setup and operation fast and simple, with a minimal learning curve.
GENERAL
| Microplate types | 6- to 1536-well plates |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides Support for labware up to 1.5″ tall |
| Software | Gen5 Microplate Reader and Imager Software included Gen5 Image+ and Image Prime Software available for advanced image analysis (option) Gen5 Secure for 21 CFR Part 11 Compliance (option) |
| X/Y Stage resolution | Lead screw driven stage with 0.1 micron resolution |
IMAGING SYSTEM
| Imaging mode | Fluorescence, high contrast brightfield and color brightfield |
| Imaging method | Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode |
| Light source | High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
| Camera | 16-bit grayscale Sony CMOS |
| Camera binning | Optional 2×2 binning for focus and/or image capture |
| Camera exposure range | 5 milliseconds to 4 seconds |
| Image outputs available | Raw Images: 16-bit TIFF Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF Movies: MP4, WMV |
| Objective capacity | 6 onboard, user-replaceable objectives |
| Objectives available | Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3 High NA: 20x, NA 0.75; 40x, NA 0.95 |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 |
| Automated functions | Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
| Autofocus method | Image-based autofocus Laser autofocus option |
| Image collection rate | Single well fastest frame rate capture: *Full Resolution: up to 10 frames per second for single color images *2×2 Binning: up to 20 frames per second for single color images |
| Microscope stage control | Gen5 Software Control Optional joystick controller |
PHYSICAL CHARACTERISTICS
| Power | 60 Watts maximum consumption. |
| Dimensions | 18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm) |
| Weight | 51 lbs (23.1 kg) |
REGULATORY
| Regulatory | CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. |
LLX
Lionheart LX Automated Microscope. Fluorescence, high contrast brightfield and color brightfield imaging. Includes standard Gen5 software and 4-position slide holder. The imaging controller, objectives, filter/LED cubes, accessories and software upgrades are sold separately.
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