Synergy Neo2
Synergy Neo2
Hybrid Multi-Mode Reader
Synergy™ Neo2 Multi-Mode Microplate Reader is designed for the screening laboratory, with speed and ultra-high performance. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF and up to 4 PMTs provide ultra-fast measurements with excellent results. For walk-away automation and high throughput, BioStack Neo is the fastest on the market, and the barcode-labeled filter cubes help streamline workflows and limit errors. Synergy Neo2 features BioTek’s patented Hybrid Technology™, with its independent optical paths that ensure uncompromised performance in all detection modes. Advanced environmental controls, including CO2/O2 control, incubation to 65 °C and variable shaking support live cell assays; cell-based detection is optimized with direct bottom illumination. Powerful Gen5 Software offers complete reader control, powerful data analysis, and automation and LIMS integration.
- Patented Hybrid Technology™ with independent filter and monochromator-based optics
- Ultra-fast plate processing speeds with multiple PMT detectors
- Two lasers: laser for TRF, TR-FRET and laser-based Alpha detection
- Variable bandwidth scientific quad monochromators for optimal sensitivity and flexibility
- Controlled environment and direct bottom detection for live cell assays
- Gen5 software for complete control and automation and LIMS integration into automation and LIMS
WHAT’S NEW
The TRF laser provides ultra-fast measurement speed, along with optimal sensitivity for critical screening applications like TRF, and TR-FRET. Laser-based detection ensures the best performance for Alpha assays.
APPLICATIONS
Synergy Neo2 is well suited for many applications requiring a variety of detection modes. Here we list some of the most important applications for this fast, high performance microplate reader.
- HTS
- Biomarker Assays
- Nucleic acid quantification
- Protein quantification
- Rapid kinetics
- HTRF
- SNP
- TR-FRET
- BRET
- Cytokines
- ELISA
- Fluorescence polarization
- Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate
hTNFα analyte titrations
Nucleic acid quantification using PicoGreen®
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
FEATURES
Patented Hybrid Technology
Hybrid Technology offers many benefits for screening and other multi-mode workflows, including the flexibility to easily select a discrete wavelength and perform spectral scans for excitation or emission peaks. Filter-based fluorescence provides fast wavelength switching and excellent sensitivity.
Multiple PMT detectors
Up to 4 PMTs are available in the highly configurable, modular Synergy Neo2, for ultra-fast measurements in all detection modes. Two top PMTs meet the requirements for ultrafast ratiometric assays.
TRF laser and Alpha laser
The TRF laser provides ultra-fast measurement speed, along with optimal sensitivity for critical screening applications like TRF, and TR-FRET. Laser-based detection ensures the best performance for Alpha assays.
Variable bandwidth monochromators
Synergy Neo2 has quadruple scientific monochromators, with the fluorescence module offering variable bandwidths from 3 nm to 50 nm, selectable in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.
Live-cell assay optimized
Temperature control to 65 °C, plus linear and orbital shaking and an available gas controller module optimize the environment in Synergy Neo2 for live cell assays. The dedicted filter-based bottom optical path directly illuminates the plate bottom, increasing the sensitivity of live cell measurements, while a dual reagent injector provides quick inject/image capability for observing fast reactions.
Gen5 Data Analysis Software
Gen5 Microplate Reader and Imager Software doesn’t compromise on usability or data analysis performance – protocol creation is intuitive and flexible, and the data analysis capability rivals standalone packages. For the GxP lab, Gen5 Secure software offers features to help comply with 21 CFR Part 11, including electronic signature, data security and multiple level permissions.
GENERAL
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Alpha |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | 6- to 1536-well plates |
| Other labware supported | Petri and cell culture dishes Take3 Micro-Volume Plates |
| Temperature control | 4-Zone incubation to 65 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Microplate Reader and Imager Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | Range: 0 – 20% (CO2); 1 – 19% (O2) Control Resolution: + 0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2%at 1% O2 Models for CO2 /O2 or CO2 only are available |
| Barcode reader | 1D and 2D camera-based scanner |
ABSORBANCE
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 230 – 999 nm, 1 nm increments |
| Monochromator bandwidth | <3 nm (230 – 285 nm), <6 nm (>285 nm) |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 well: 6 seconds |
FLUORESCENCE INTENSITY
| Light source | Xenon flash |
| Detector | Dual top PMTs Single top PMT (option) Low noise PMT (bottom filter system) Red shifted PMT (top/bottom monochromator system) |
| Wavelength selection | Quad monochromators (top/bottom) Filters (top/bottom) |
| Wavelength range | Monochromators: 250 – 850 nm Filters (dual PMT): 200 – 850 nm |
| Monochromator bandwidth | Variable, from 3 to 50 nm in 1 nm increments |
| Dynamic range | 7 decades |
| Sensitivity | Filters: Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) – top Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) – top Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) – bottom Quad monochromator: |
| Reading speed (kinetic) | 96 well: 6 seconds 384 well: 11 seconds 1536 well: 25 seconds |
LUMINESCENCE
| Wavelength range | 300 – 700 nm |
| Dynamic range | > 6 decades |
| Sensitivity | 5 amol ATP typical (384-well low volume plate) |
FLUORESCENCE POLARIZATION
| Light source | Xenon flash |
| Detector | Dual PMT or single PMT (option) |
| Wavelength selection | Filters |
| Wavelength range | 280 – 850 nm |
| Sensitivity | 1 mP standard deviation at 1 nM fluorescein |
TIME-RESOLVED FLUORESCENCE
| Light source | Xenon flash or TRF laser (option) |
| Detector | Dual PMT or single PMT (option) |
| Wavelength selection | Filters (top/bottom) |
| Wavelength range | Monos: 250 – 850 nm Filters (dual PMT): 200 – 850 nm |
| Sensitivity | With TRF laser: 5 fM (384-well low volume plate) With Xenon flash lamp: 40 fM (384-well low volume plate) |
ALPHA DETECTION
| Light source | 100 mW 680 nm laser |
| Detector | PMT |
| Wavelength selection | Filters (top) |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
| Read speed | 96 well: 30 seconds 384 well: 1 minute 50 seconds 1536 well: 7 minutes 20 seconds |
REAGENT DISPENSERS
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 – 1000 µL in 1 µL increments |
| Dispense accuracy | + 1 µL or 2% |
| Dispense precision | < 2% at 50-200 µL |
PHYSICAL CHARACTERISTICS
| Power | 250 Watts maximum consumption. |
| Dimensions | Without TRF laser: 15.4″ W x 20.7″ D x 16.1″ H (39.2 x 52.5 x 40.8 cm) With TRF laser: |
| Weight | Without TRF laser: 78 lbs. (35.4 kg) With TRF laser: 100 lbs. (45.3 kg) |
REGULATORY
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
| Monochromator | Filter | ||||||
|---|---|---|---|---|---|---|---|
| ABS | FI and LUM | Dual filter top FI/FP/TRF and LUM | Single filter top FI/FP/TRF and LUM | Filter bottom FI/FP/TRF | Alpha laser | TRF laser | |
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