405 LS
405 LS
Washer
The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability for washing protocols ranging from gentle cell washing to vigorous ELISAs. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.
- Intuitive onboard User Interface or LHC™ pc software
- Magnetic and polystyrene bead assays, conventional ELISAs
- Dual-Action™ manifold
APPLICATIONS
Beyond standard dispense/aspirate protocols, the 405 LS automates liquid handling functions for many applications, and is ideal for integration into automated systems, controlled with LHC software.
- ELISA
- Bead washing
- Cell washing /media exchange
Fluorescence calibration curves for a 13-plex human metabolic hormone panel, using magnetic microspheres washed on the 405.
FEATURES
Intuitive onboard User Interface or LHC™ pc software
The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions.
Magnetic and polystyrene bead assays, conventional ELISAs
The 405 LS is ideal for many standard plate washing and dispensing protocols, but it also automates more complex operations like magnetic and polystyrene bead washing workflows – and it’s Luminex® xMap® approved. Bead washing is available for 96- and 384-well microplates.
Dual-Action™ manifold
With its angled dispense tubes and adjustable flow rates, the 405 LS can process sensitive cell-based assays. The optional Dual-Action manifold and carrier offer X-Y-Z adjustment, perfect for optimizing plate positioning and residual removal for cell-based and other assays.
GENERAL
| Microplate types | 96- and 384-well Low profile and standard height Solid and filter bottom (filter pore sizes 0.45 µm to 1.2 µm) |
| Onboard software | Create, edit or run multiple protocols |
| Software (computer control) | LHC2 pc software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) |
| Separation | Biomagnetic separation, vacuum filtration (optional) |
| Shaking | Programmable up to 60 minutes Slow, medium, fast or variable |
| Soaking | Programmable up to 60 minutes |
| Ultrasonic Advantage™ | Yes (option) |
| Verify™ clog detection | Yes (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
WASHING
| Manifold types | 96-well washing: 96-tube manifold – straight dispense tubes 96- and 384-well washing: 96-tube Dual-Action manifold – 20° angled dispense tubes 384-well washing (fast): 192-tube Dual-Action manifold – 7° angled dispense tubes |
| Volume range | 25 to 3,000 µL/well, in 1 µL increments |
| Wash cycles | 1 – 250 |
| Buffer/reagent selection | Auto switching (internal) for up to 4 buffers (option) |
| Supply bottle | 4 L or 10 L (optional) |
| Dispense precision | <3% CV: 300 µL/well (96-well washing) <4% CV: 80 µL/well (384-well washing) |
| Residual volume | <2 µL/well (96- & 384-well plates) 96-tube manifold for 96 wells; 192-tube for 384 wells |
| Wash speed | 96-wells, 300 µL/well, 3 cycles; <30 seconds 384-wells,100 µL/well, 3 cycles: <80 seconds 384-wells, 400 µL/well, 1 cycle: <20 seconds |
| Flow rates | High flow to low flow Optimized rates for cell assays |
| Sterilization | Chemical |
| Vacuum filtration | Selectable: Approximate final at 30 seconds: Lowest: -38 mmHg Low: -113 mmHg Medium: -200 mmHg High: -390 mmHg Highest: -506 mmHg Vacuum filtration time range: 5 to 999 seconds |
PHYSICAL CHARACTERISTICS
| Power | 100 – 240 Volts AC. 50/60 Hz |
| Dimensions | 14″ W x 17″ D x 10″ H (35.6 x 43.2 x 25.4 cm) |
| Weight | With internal buffer switching – 36 lbs (16.5 kg) Without – 30 lbs (13.5 kg) |
REGULATORY
| Regulatory | CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available. |
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Part #
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384-well
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96-well
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Optimized Cell Washing
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4-Buffer Switching
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Biomagnetic Separation**
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Vacuum Filtration**
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* 96-well washing available with optional manifold PN 1170010, sold separately
** With available accessory module sold separately
| 405™ LS Microplate Washer Configurations | ||
|---|---|---|
| Feature | Design | |
| HT | Faster 384-well microplate washing |
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| V | Automated Internal 4-Buffer Switching | |
| S | Ultrasonic Advantage™ |
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| U | 96-/384-well microplate washing |
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| R | 96-well microplate washing |
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| Q | Verify™ Technology |
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