Cytation Hybrid
Cytation
Hybrid Multi-Mode Reader
Cytation Multi-Mode readers offer modularity to meet your laboratory’s present workflows, and are upgradable to meet future requirements. UV-Vis absorbance, fluorescence and luminescence detection are available detection modes, and you can add fluorescence microscopy to expand the range of applications in a single instrument. The included Gen5™ software is designed for uncomplicated data collection and processing for even the most complex assays.
- Modular and upgradable to meet current and future workflow requirements
- Patented Hybrid Technology™ optics with the sensitivity of filters and flexibility of monochromators
- Variable bandwidth monochromators optimize fluorophore detection
- 100 mW laser for speed and performance-based excitation for Alpha assays
- Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate
- Peltier Cooling Module maintains environmental stability for uncompromised assay integrity
APPLICATIONS
Cytation Cell Imaging Multi-Mode Readers are uniquely designed to support applications across automated digital microscopy and conventional multi-mode detection. Here we highlight a few key application areas.
- Cell based assays
- Biomarker assays
- Imaging applications
- Protein quantification
- Kinetic assays
- Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate
Standard curves compared; Cytation 5 and Synergy HTX AlphaLISA assay
Fluorescence polarization: Human serum albumin and tryptophan
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
FEATURES
Modular, upgradable design
Although your lab may only be working with multi-mode workflows now, assay demands may change in the future. Cytation’s modularity offers the ability to add automated digital microscopy to the multi-mode system to adapt as your research requirements change.
Patented Hybrid optics
Cytation 5 features a patented, hybrid optical design, with filter-based fluorescence optics for high transmission and high sensitivity, and monochromator optics for convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization.
Variable bandwidth monochromators
Cytation 5 features monochromators with variable bandwidths from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.
100 mW Alpha Laser
Laser-based excitation in Cytation 5 offers the power required to get great sensitivity and fast reading speeds, which are critical for Alpha assays.
Peltier Cooling for environmental stability
The Peltier Cooling Module promotes a rapid interior cool down after incubated processes, to allow efficient switching between multiple applications without unwanted temperature influences. For temperature sensitive assays typically run at ambient, or room temperature, the Cooling Module maintains environmental stability within Cytation, allowing < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation.
GENERAL
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Alpha |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | Monochromator: 6- to 384-well plates Filters: 6- to 1536-well plates Imaging: 6- to 1536-well plates |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates |
| Temperature control | 4-Zone incubation to 65 °C with Condensation Control; +0.2 °C at 37 °C |
| Cooling | Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides rapid cooling after incubated processes. |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Microplate Reader and Imager Software Gen5 Image+ and Image Prime software available for full image analysis Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | Range: 0 – 20% (CO2); 1 – 19% (O2) Models for both CO2 and O2 or CO2 only are available |
IMAGING SYSTEM
| Imaging mode | Fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast |
| Imaging method | Single color, multi-color, montage, time lapse, z-stacking |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | 16-bit Sony CMOS, standard or wide FOV |
| Objective capacity | 6 user-replaceable objectives |
| Objectives available | 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x |
| Phase objectives available | 4x, 10x, 20x, 40x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Automated functions | Autofocus, auto LED intensity, auto exposure |
| Autofocus method | Image-based autofocus User-trained autofocus Laser autofocus (option) |
| Positional controls | Software control Joystick control (option) |
| Image collection rate | Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutes Laser autofocus: |
| Image Analysis Software option | Gen5 Image+: Advanced image analysis Gen5 Image Prime: Advanced image analysis Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features |
FLUORESCENCE INTENSITY
| Light source | Xenon flash |
| Detector | PMT for monochromator system PMT for filter system |
| Wavelength selection | Quad monochromators (top/bottom) Filters (top) |
| Wavelength range | Monochromators: 250 – 700 nm (850 nm option) Filters: 200 – 700 nm (850 nm option) |
| Monochromator bandwidth | Variable, from 9 nm to 50 nm in 1 nm increments |
| Dynamic range | 7 decades |
| Sensitivity | Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) Quad Monochromator: |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
LUMINESCENCE
| Wavelength range | 300 – 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | Monos: 20 amol ATP (flash) Filters: 10 amol ATP (flash), 100 amol (glow) |
FLUORESCENCE POLARIZATION
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 – 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nM fluorescein |
TIME-RESOLVED FLUORESCENCE
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (secondary mode) Filters (top) |
| Wavelength range | Filters: 200 – 700 nm (850 nm option) |
| Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
ABSORBANCE
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | Monochromator |
| Wavelength range | 230 – 999 nm, 1 nm increment |
| Monochromator bandwidth | 4 nm (230 – 285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
ALPHA DETECTION
| Light source | 100 mW 680 nm laser |
| Detector | PMT |
| Wavelength selection | Filters (top) |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
REAGENT INJECTORS (OPTION)
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 – 1000 µL in 1 µL increments |
| Plate geometry | 6- to 384-well microplates, Petri dishes |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 – 200 µL |
PHYSICAL CHARACTERISTICS
| Power | 250 Watts maximum consumption. |
| Dimensions | 20″ D x 16.5″ W x 17.5″ H (50.8 cm x 41.91 cm x 44.5 cm) |
| Weight | 80 lbs (36.3 kg) |
REGULATORY
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
Imaging Plus Multi-Mode Configurations
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CYT5FV
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CYT5FW |
CYT5FAV
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CYTFAW |
CYT5MV
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CYT5MW |
CYT5MPV
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CYT5MPW |
CYT5MFV
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CYT5MFW | CYT5MFAV | CYT5MFAW |
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Fluorescence imaging
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Brightfield imaging
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Color brightfield imaging
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Phase contrast imaging
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Wide FOV camera
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Filter-based fluorescence intensity
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Filter-based fluorescence polarization
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Filter-based time resolved fluorescence
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Filter-based luminescence
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Filter-based Alpha detection
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Monochromator-based UV-Vis absorbance
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Monochromator-based fluorescence intensity
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Monochromator-based time resolved fluorescence (secondary)
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Monochromator-based Luminescence
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Take3 Plate compatible
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Imaging Only and Multi-Mode Only Configurations
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CYT5V
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CYT5W |
CYT5PV
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CYT5PW | CYT5MF | CYT5MFA | CYT5F | CYT5FA | CYT5M |
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Fluorescence imaging
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Brightfield imaging
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Phase contrast imaging
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Wide FOV camera
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Filter-based fluorescence intensity
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Filter-based
fluorescence polarization
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Filter-based time resolved fluorescence
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Filter-based luminescence
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Filter-based Alpha detection
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Monochromator-based UV-Vis absorbance
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Monochromator-based Fluorescence intensity
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Monochromator-based Time resolved fluorescence (secondary)
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Monochromator-based Luminescence
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Take3 Plate compatible
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All configurations include incubation to 65 °C and shaking, and are compatible with BioTek’s Dual Reagent Injection Module, CO2/O2 Gas Controller, and BioStack Microplate Stacker, and BioSpa Automated Incubator.
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