Synergy H1
Synergy H1
Hybrid Multi-Mode Reader
Synergy™ H1 is a configurable multi-mode microplate reader. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements.
- Cost-effective hybrid offering excellent sensitivity, flexibility and value
- Modular and upgradable: choose the modes you need now, add as needs change
- Patented Hybrid Technology: sensitivity of filters and flexibility of monochromators
- Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate
- Live cell assay friendly with temperature control and CO2/O2 control
APPLICATIONS
With quadruple monochromators and optional gas control and reagent injection, Synergy H1 can be used for a wide range of applications in fluorescence, luminescence, UV-Vis and more.
- Cell based assays
- Biomarker Assays
- Micro-volume analysis of nucleic acid and protein
- Kinetic assays
- Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate
Effect of increasing levels of ethanol on yeast growth
HTRF® ratios for AMD3011 and SDF1-alpha
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
FEATURES
Cost-effective hybrid
Modular, upgradable design
With six different base configurations to choose from, Synergy H1 is an affordable, upgradable solution to address your laboratory’s research needs now and well into the future.
Patented Hybrid optics
Synergy H1’s hybrid optical design offers filter-based fluorescence optics for high transmission and high sensitivity, while the available monochromator optics offers convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization
Micro-volume quantification
The Take3 Micro-Volume plate extends the Synergy H1 capabilities beyond standard microplate reading, by measuring up to 48, 2 µL samples at one time for rapid nucleic acid and protein quantification. Absorbance and fluorescence measurements are quick and easy, no additional instrumentation is required!
Live cell assay friendly environment
Synergy H1 offers temperature control to 45 °C, plus linear and orbital shaking. Expand the applications base to live cell assays by adding the CO2/02 controller or the dual reagent injector for quick inject/read processes.
TECHNICAL DETAILS
GENERAL
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported | Petri and cell culture dishes Take3 Micro-Volume Plates |
| Temperature control | 4-Zone™ incubation to 45 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Data Analysis Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) | Range: 0 – 20% (CO2); 1 – 19% (O2) Control Resolution: +0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2% at 1% O2 Models for both CO2 and O2 or CO2 only are available |
ABSORBANCE
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 230 – 999 nm, 1 nm increments |
| Monochromator bandwidth | 4 nm (230-285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | ±2 nm |
| Monochromator wavelength repeatability | ±0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
FLUORESCENCE INTENSITY
| Light source | Xenon flash |
| Detector | PMT for monochromator system PMT for filter system |
| Wavelength selection | Quad monochromators (top/bottom) Filters (top) |
| Wavelength range | Monochromators: 250 – 700 nm (850 nm option) Filters: 200 – 700 nm (850 nm option) |
| Monochromator bandwidth | Fixed, 16 nm |
| Dynamic range | 7 decades |
| Sensitivity | Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) Quad Monochromator: |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
LUMINESCENCE
| Wavelength range | 300 – 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | Monos: 20 amol ATP (flash) Filters: 10 amol ATP (flash), 100 amol (glow) |
FLUORESCENCE POLARIZATION
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 – 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nm fluorescein |
TIME-RESOLVED FLUORESCENCE
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (secondary mode) Filters (top) |
| Wavelength range | Filters: 200 – 700 nm (850 nm option) |
| Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
REAGENT DISPENSERS
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 – 1000 µL in 1 µL increment |
| Dispense accuracy | ±1 µL or 2% |
| Dispense precision | <2% at 50-200 µL |
PHYSICAL CHARACTERISTICS
| Power | 130 Watts maximum consumption. |
| Dimensions | 15.4″W 18.6″D 12.9″H (39.1 x 47.2 x 32.8 cm) |
| Weight | 50 lbs (22.5 kg) |
REGULATORY
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
CONFIGURATIONS
|
Part #
|
H1M
|
H1F
|
H1MF
|
|---|---|---|---|
|
Monochromator fluorescence
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•
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•
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Monochromator absorbance
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•
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•
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Full-light luminescence
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•
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•
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•
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Filter/dichroic fluorescence
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•
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•
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Fluorescence polarization
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•
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•
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Time resolved fluorescence
|
• *
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•
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•
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Filtered luminescence
|
•
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•
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Temperature control to 45°C
|
•
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•
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•
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Gen5 data analysis software
|
•
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•
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•
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Gas Controller Compatible Configurations**
|
H1MG | H1FG | H1MFG |
* Secondary mode
** These configurations have the same features as the non- Gas Controller Compatible configurations in the chart above. Gas Controller sold separately.
All configurations are compatible with the Dual Reagent Injector Module, sold separately.
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