Epoch 2
Epoch 2
Microplate Spectrophotometer
Epoch™ 2 Microplate Spectrophotometer offers excellent performance for UV-Vis measurements in 6- to 384-well microplates, cuvettes and in micro-volume samples with the available Take3™ Micro-Volume Plate. The optional touchscreen interface makes it easy to choose from pre-defined protocols or to define custom programs. Endpoint, kinetic, spectral scanning and well area scanning modes, plus incubation and shaking enable wide-ranging applications.
- Filter-free UV-Vis wavelength selection from 200 – 999 nm
- End point, kinetic and spectral scanning modes
- Compatible with 6- to 384-well plates and cuvettes for assay versatility
- 4-Zone™ incubation to 65 °C and Condensation Control™
- Micro-volume (2 µL) measurements with the Take3 Plate
- Available touchscreen interface and onboard software
APPLICATIONS
Incubation to 65 °C, plus shaking and UV-Vis absorbance range in Epoch 2 make it appropriate for many colorimetric and nucleic acid and protein quantification applications.
- Cellular and microbial growth
- ELISA
- Micro-volume analysis of nucleic acid and protein
- Kinetic assays
Glucose production from cellulosic feedstock
Nucleic acid quantification in cuvette with Epoch 2
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
FEATURES
Filter-free UV-Vis wavelength selection from 200 – 999 nm
The monochromator-based optics in Epoch 2 allows selection of wavelengths in 1 nm increments – no need to purchase filters! The broad wavelength range enables applications from nucleic acid and protein quantification in the low UV to microbial growth assays, and more.
End point, kinetic and spectral scanning
The onboard software in Epoch 2 enables several read modes including single and dual wavelength end point and kinetic measurements. Spectral scanning provides a convenient, quick spectrum of all defined samples. Under Gen5 software control, well area scanning is also available, along with powerful data analysis.
Compatible with 6- to 384-well plates and cuvettes for assay versatility
The optional cuvette port enables quick and easy 1 cm sample measurements, making Epoch 2 a versatile choice for assay workflows in a variety of labware.
4-Zone™ incubation to 65 °C and Condensation Control™
Some important applications like biofuels research and food & beverage safety and quality call for higher incubation temperatures than typically expected. Epoch 2 can meet these assay requirements. Kinetic assays are optimized with Condensation Control to prevent interference caused by condensation on plate lids.
Micro-volume (2 µL) measurements with the Take3 Plate
The Take3 Micro-Volume plates expand Epoch 2’s capabilities to measure 2 µL samples for direct DNA, RNA and protein quantification. Get rapid results for up to 48 samples at a time without additional instrumentation.
Available touchscreen interface and onboard software
Access several common end point, kinetic or micro-volume assays through the convenient touchscreen. Displayed data can be output to a printer or USB flash drive for added flexibility. Epoch 2 comes with Gen5 software for additional powerful data analysis.
TECHNICAL DETAILS
GENERAL
| Detection modes | UV-Vis absorbance |
| Read methods | Endpoint, kinetic, absorbance spectral scanning (onboard software and under Gen5 control) Endpoint, kinetic, absorbance spectral scanning, well area scanning (under Gen5 control) |
| Microplate types | 6- to 384-well plates |
| Other labware supported | Take3 Micro-Volume Plates (onboard software) Take3 and Take3 Trio Micro-Volume Plates (under Gen5 control) Standard cuvette (option) |
| Temperature control | 4-Zone™ incubation to 65 °C with Condensation Control Uniformity + 0.2 °C at 37 °C |
| Shaking | Linear, orbital, double orbital |
| Software | Onboard software with easy programming and several predefined protocols (touchscreen configurations) Full reader control and data analysis and reporting under Gen5 Software control (Gen5 Software included) |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
ABSORBANCE
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 200 – 999 nm, selectable in 1 nm increments |
| Monochromator bandwidth | 2.9 nm |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.001 OD (onboard software) 0.0001 OD (under Gen5 control) |
| Pathlength correction | yes (under Gen5 Control) |
| Monochromator wavelength accuracy | + 2 nm |
| Monochromator wavelength repeatability | + 0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 2.5 OD |
| OD linearity | <1% from 0 to 2.5 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells sweep read: 8 seconds 384 wells sweep read: 14 seconds |
PHYSICAL CHARACTERISTICS
| Power | External 24 VDC power supply compatible with 100-240 volts AC at 50-60Hz. 60 W maximum consumption. |
| Dimensions | Touch screen models: 12.5” H x 12.75” W x 15.5” D (32 x 32.3 x 39.54 cm) Non-touch screen models approximately 8.5” H x 12.75” W x 15.5” D (22 x 32.3 x 39.54 cm) |
| Weight | 25 lbs (11.34 kg) |
REGULATORY
| Regulatory | CE and TUV marked, RoHS compliant. Models for In Vitro Diagnostic use are available. |
CONFIGURATIONS
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Incubation
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Shaking
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Gen5 Software
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Touchscreen
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Cuvette port
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