MultiFlo FX
MultiFlo FX
Multi-Mode Dispenser
MultiFlo™ FX is an automated multi-mode reagent dispenser for 6- to 1536-well microplates. MultiFlo FX incorporates several unique technologies in its modular design, such as Parallel Dispense, Random Access Dispense and the new, patent-pending Automated Gentle Media Exchange modules to facilitate a variety of liquid handling applications from 2D and 3D cell culture to concentration normalization assays, ELISA, bead-based assays and more. A fully configured MultiFlo FX replaces up to five liquid handlers, saving space, time and instrumentation budgets. MultiFlo FX integrates to the BioSpa™ 8 Automated Incubator and a BioTek imager or multi-mode reader, for complete workflow automation for many cell imaging and biochemical applications.
- Multi-mode dispensing replaces multiple instruments
- Automated, gentle media exchange and spheroid washing
- Mapped dispensing to individual wells for normalization
- Wash module for 6- to 384-well plates
- Parallel Dispense™ technology: peristaltic and/or syringe pumps
- Cell friendly angled dispense and/or wash tubes
WHAT’S NEW
Now with Automated, Gentle Media Exchange (AMX) and Custom-mapped RAD Dispensing!
MultiFlo FX – AMX for Gentle
Automated Media Exchange
MultiFlo FX – RAD Mapped Dispensing
APPLICATIONS
This compact, versatile dispenser enables a wide variety of applications for benchtop and integrated automation workflows.
- Bulk reagent dispensing
- Cell seeding
- Cell washing /media exchange
- ELISA automation
- Bead washing
- Concentration normalization assays
Import custom RAD plate maps for concentration normalization assays
The configurable MultiFlo FX: 4 reagent dispensers, washer, media exchange and single channel dispenser
FEATURES
Multi-mode dispensing replaces multiple instruments
Providing unsurpassed liquid handling flexibility, MultiFlo FX’s modular base can accommodate 1 or 2 peristaltic pumps, a 2-syringe pump module and a wash module or a single channel dispense. MultiFlo FX takes the place of multiple instruments, but is compact and very simple to use.
Automated, gentle media exchange for spheroids
Mapped dispensing to individual wells
Import custom .CSV file for RAD dispensing
The unique RAD technology enables custom-mapped dispensing to individual wells to facilitate many applications including concentration normalization assays.
Wash module for 6- to 384-well plates
The versatile wash module for MultiFlo FX enables 6- to 384-well washing with custom manifolds. Dispensing and aspirate tube positioning, fluid flow rate and wash parameters are fully customizable, and the angled dispense tubes provide gentle washing for cell-based assays. The module uses integrated dispense and waste pumps to keep the system compact.
Parallel Dispense™ technology: peristaltic and/or syringe pumps
Peristaltic pumps and syringe pumps each offer benefits; peri-pumps help save precious reagent, while syringe pumps are fast and precise. MultiFlo FX can be configured with 2 peri and 2 syringe pumps, allowing up to four reagents to be dispensed in parallel, without carryover.
Cell friendly angled dispense and/or wash tubes
For sensitive cell-based assays, the dispense tips on the MultiFlo FX dispense manifolds and wash manifolds are angled, allowing gentle reagent dispense down the well sides. This helps keep cell layers intact, even through multiple-step operations.
GENERAL
| Microplate types | Dispensing (peri pump and syringe) 96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable) Washing 96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration) |
| Onboard software | Create, edit or run multiple protocols |
| Software (computer control) | LHC2 Software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) |
| Shaking | Programmable up to 60 minutes Slow, medium, fast or variable |
| Soaking | Programmable up to 60 minutes |
| USB ports (2) | For protocol storage/transfer and for optional external mouse or keyboard |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
WASHING
| Manifold types | 96- and 384-well washing: 8-tube manifold 6-, 12-, 24-, 48-well washing: Custom manifolds available |
| Volume range | 20-30,000 µL/well |
| Wash cycles | 1-10 |
| Supply bottle | 2 L |
| Dispense accuracy | +3% |
| Dispense precision | 96-/384-well plates, 300 µL/well: <3% CV 6-well plates, 5560 µL/well: <5% CV |
| Residual volume | 96-well plate, 300 µL/well: < 2 µL/well |
| Wash speed | 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds |
| Wash volume | 20-30,000 µL/well |
| Waste bottle | 2L |
| Flow rates | 140-422 µL/well/second |
| Sterilization | Chemical |
DISPENSING – PERISTALTIC PUMP (MULTI-CHANNEL)
| Manifold types | 1 x 8 – sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing |
| Fluid delivery | 1 or 2 peristaltic pumps |
| Dispense speed | 96 wells, 5 µL cass, 10 µL/ well: 3 seconds 96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds 384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds 384 wells, 10 µL cass, 10 µL/ well: 8 seconds 384 wells, 1 µL cass, 1 µL/ well: 6 seconds 1536 wells, 1 µL cass, 1 µL/ well: 21 seconds |
| Volume range | 500 nL – 3,000 µL/well, selectable in 1 µL increments |
| Flow rates | User programmable rates from high to low |
| Dispense performance | 1 µL cassette: recommended range: 1 – 50 µL Accuracy: +5% at 1 µL Precision: <5% CV at 1 µL <10% CV at 500 nL Minimum Prime Volume: 1.20 mL 5 µL cassette: recommended range: 5 – 2,500 µL 10 µL cassette: recommended range: 10 – 3,000 µL |
| Recommended cassette replacement interval | 1 µL Cassette: 1,000 384-well microplates at 5 µL well 5 µL Cassette: 1,000 96-well microplates at 50 µL well 10 µL Cassette: 1,000 96-well microplates at 100 µL well |
| Sterilization | Autoclave, chemical |
DISPENSING – SYRINGE PUMP
| Manifold types | 96- and 384-well dispensing: One 16-tube (2 x 8) manifolds – 316 SS tubes Two 16-tube (1 x 16) manifolds – 316 SS tubes 1536-well dispensing: |
| Fluid delivery | Two positive displacement syringe drives |
| Dispense speed | 20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds 20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds 3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds |
| Volume range | 3 – 3,000 µL/well selectable in 1 µL increments Minimum Prime Volume: 12 mL |
| Flow rates | User programmable rates from high to low |
| Dispense accuracy | +1 µL at 5 µL +1 µL at 20 µL +1% at 100 µL |
| Dispense precision | <5% CV at 5 µL <2.5% CV at 20 µL <1% CV at 100 µL |
| Supply bottle | 1 L or 2 L |
| Sterilization | Chemical, autoclavable option |
DISPENSING: RAD (RANDOM ACCESS MODULE)
| Labware types | Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile standard height and deep well formats; PCR trays and microtubes 8-to-1 tip: 6-, 12-, and 24-well plates |
| Manifold Types | RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute |
| Volume range | 500 nL – 30,000 µL |
| Minimum prime volume | 1 µL cass, 18″: 90 µL ; 1 µL cass, 30″: 150 µL 5 µL cass, 18″: 320 µL; 1 µL cass, 30″: 530 µL 10 µL cass, 18″: 555 µL; 10 µL cass, 30″: 920 µL |
| Dispense speed (high flow) | 1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells) 1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells) 5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells) 5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells) 10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells) 10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells) |
| Dispense performance | 1 µL cass (med), 0.5 µL/well: Precision 10% CV 1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV 1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV 5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV 5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV 10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV 10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV 8-to 1 cass(high), 40 µL/well: Accuracy + 4% 8-to 1 cass (high), >10 µL/well: Precision 2.5%CV 8- to 1cass(high), >80 µL/well: Accuracy + 2% |
MEDIA EXCHANGE: AUTOMATED MEDIA EXCHANGE MODULE
| Microplate types | 96- and 384-well standard (ANSI/SBS) |
| Manifold types | Two 8-channel autoclavable manifolds, tube-in-tube design |
| Cassettes | Autoclavable cassettes with with 5 µL tubing |
| Dispense Precision | <5% CV |
| Dispense Accuracy | <5% |
| Aspiration uniformity | <5% |
| Sterilization method | Cassettes and manifolds: chemical and autoclavable |
PHYSICAL CHARACTERISTICS
| Power | 100 – 240 Volts AC. 50/60 Hz |
| Dimensions | Base instrument: 17.19″W x 11.75″ D x 8″ H (43.51 x 29.21 x 20.32 cm) |
| Weight | Base instrument: 19.5 lbs (8.8 Kg) |
REGULATORY
| Regulatory | CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available. |
| Part # | Description |
|---|---|
|
MFXP1
|
MultiFlo FX with 1 module arm, 1 peristaltic dispense pump, 1 peri-pump cassette with plastic tips. |
|
MFXP2
|
MultiFlo FX with 2 module arms, 1 peristaltic dispense pump. Includes 1 peri-pump cassette with plastic tips. |
|
MFXP2R
|
MultiFlo FX with RAD single channel dispenser. Includes 2 module arms, 2 peristaltic dispense pumps, 1 RAD manifold, and tubing included. |
Optional Modules
| Compatible With | MFXP1 | MFXP2 | MFXP2R | |
|---|---|---|---|---|
|
7210010
|
Secondary peripump module. Includes module and choice of (1) one peri-pump cassette with plastic tips. | • | • | |
|
7210008
|
Dual syringe pump module (autoclavable). Includes module with two syringe pumps, (2) autoclavable bottles (PN 7140511) and (1) autoclavable tubing set (PN 7183006). Manifold(s) are required and must be purchased separately, see “Syringe Pump Dispenser Module” section for available manifolds. | • | • | • |
|
7210009
|
Dual syringe pump module (non-autoclavable). Includes module with two pumps, (2) non-autoclavable bottles (PN 4070515) and (1) non-autoclavable tubing set (PN 7183002). Manifold(s) are required and must be purchased separately, see “Syringe Pump Dispenser Module” section for available manifolds. | • | • | • |
|
1260023
|
Wash module. Includes pump, bottles, tubing and one 96/384 wash manifold. Other manifolds available, order separately. | • | • | |
|
1260041
|
AMX Automated Media Exchange module. Includes secondary peri pump module, AMX dispense and aspirate manifolds. Two cassettes (PN 1260035) are required, order separately. | • |
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