PowerWave HT
PowerWave HT
Microplate Spectrophotometer
With super-fast plate reading speeds and a broad UV-Vis wavelength range, BioTek’s PowerWave HT Microplate Spectrophotometer is ideal for myriad applications, from direct quantification of nucleic acids to full-scale high throughput operations that demand speed, accuracy and reliability. Rugged hardware, proven optical performance and a very small footprint, the PowerWave HT is uniquely designed to provide the most application flexibility while saving space both on the benchtop and in automated systems.
- 200 nm – 999 nm with no interference filters required
- Fast 96- and 384- well plate reading
- Superior 4-Zone™ temperature control
- Compact footprint well suited for automation
- Gen5 Microplate Reader and Software for data collection and powerful analysis
APPLICATIONS
Fast kinetics and UV-Vis applications are the focus for PowerWave HT, for benchtop or integration into automated systems. 4-Zone™ Incubation and shaking add to the capabilities of this compact, high-performing microplate spectrophotometer.
- Cellular and microbial growth
- ELISA
- Nucleic acid quantification
Kinetic ß-galactosidase assay
FEATURES
No Interference Filters Required
Monochromator optical design provides continuous wavelength selection from 200 nm to 999 nm. Measuring wavelengths can be selected in 1 nm increments to meet all assay requirements – it’s like having 800 filters built in! Spectral scanning capability allows quick determination of peak wavelengths in unknown solutions.
Microplate Types
96 and 384-well plates measured. In addition, the PowerWave HT can also read up to 8 of BioTek’s patented Bio-Cell™ quartz vessels for fixed 1 cm measurements.
Fast Reading Speed
Using “on the fly” technology, an entire 96-well plate can be read as few as 5 seconds.
Temperature Control and Microplate Shaking
BioTek’s 4-Zone™ temperature control is a standard feature with the PowerWave HT. BioTek microplate readers use a natural convection system rather than a forced hot air system. This reduces evaporation in the microplate across its broad temperature range of 4° above ambient to 50°C. In the reading chamber, the microplate is completely surrounded by four independently controlled heaters ensuring low variation across the microplate. Users select from four shaking intensities and can define shake time for controlled agitation.
Robotics Compatible
The PowerWave HT’s small footprint makes it ideal for robotic systems. The carrier is designed for easy robot gripper access. PowerWave HT is compatible with BioStack 3 and BioStack.
Gen5™ Data Analysis Software included with all models.
GENERAL
| Detection modes | Absorbance |
| Read methods | Endpoint, kinetic, spectral scanning |
| Microplate types | 96- and 384-well plates |
| Temperature control | 4-Zone™ incubation to 50 °C + 0.2 °C at 37 °C |
| Shaking | Linear |
| Software | Gen5™ Data Analysis Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible |
ABSORBANCE
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 200 to 999 nm, in 1 nm increments |
| Monochromator bandwidth | 5 nm |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | 1% + 0.01 OD |
| OD linearity | +1% |
| OD repeatability | 0.5% + 0.005 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 5 seconds 384 wells: 11 seconds |
PHYSICAL CHARACTERISTICS
| Power | 100-240 Volts AC. 50/60 Hz. |
| Dimensions | 8.5″W x 16″D x 8.5″H (21.6 x 40.6 x 21.6 cm) |
| Weight | 24 lbs (11 kg) |
REGULATORY
| Regulatory | CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available. |
RPRWI
PowerWave HT: 200-999 nm, 96- and 384-well plate reading with programmable shaking and temperature control from ambient +4°C to 50°C.
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