Synergy HTX
Synergy HTX
Multi-Mode Reader
Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. It’s unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence and AlphaScreen®/ AlphaLISA® workflows. Incubation and shaking plus a dual reagent injector module to meet all your laboratory’s assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 Software for data collection, analysis, exporting and reporting.
- Great flexibility at a low price
- Monochromator – based UV/Vis absorbance and filter-based fluorescence
- Linear and orbital shaking support and optimize many applications
- Excellent performance with AlphaScreen® and AlphaLISA®
- Dual Reagent Injectors are ideal for inject/read assays
- Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate
APPLICATIONS
With UV-Vis absorbance and filter-based fluorescence and luminescence, Synergy HTX combines versatility and performance for many key endpoint and kinetic applications.
- Cell based assays
- Biomarker Assays
- Kinetic assays
- Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate
Electron structures of reactive oxygen curve (ROS) species, detectable using Synergy HTX
Gen5 calculated area under the curve (AUC) for an ORAC assay.
Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
FEATURES
Great flexibility at a low price
Monochromator and filter optics
Shaking for assay optimization
Excellent Alpha assay performance
Fast inject/read assays
The available dual reagent injector module with angled tips, enables fast inject/read operations, which is critical for enzyme kinetics, Dual-Luciferase® Reporter and many other assays. Synergy HTX is easily upgradable to add the dual reagent injector.
GENERAL
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Time-resolved fluorescence (secondary mode) Alpha |
| Read methods | End point, kinetic, spectral scanning and well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported | PCR plates, Petri and cell culture dishes Take3 Micro-Volume Plates |
| Temperature control | 4-Zone™ incubation to 50 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital |
| Software | Gen5™ Microplate Reader and Imager Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation | BioStack and 3rd party automation compatible |
ABSORBANCE
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 200 – 999 nm, 1 nm increments |
| Monochromator bandwidth | 2.4 nm |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD <3% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 14 seconds 384 wells: 26 seconds |
FLUORESCENCE INTENSITY
| Light source | Tungsten halogen Xenon flash (option) |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 300 – 700 nm (200 – 850 nm option) |
| Dynamic range | >6 decades |
| Sensitivity | Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate) |
| Reading speed (kinetic) | 96 wells: 31 seconds 384 wells: 80 seconds |
LUMINESCENCE
| Wavelength range | 300 – 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | 10 amol ATP (flash) – Lum and Abs / Lum configurations 30 amol ATP (flash) – Multi-mode configurations |
TIME-RESOLVED FLUORESCENCE
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Monochromator |
ALPHA DETECTION
| Light source | Tungsten halogen |
| Detector | PMT |
| Wavelength selection | Filters |
| Sensitivity | 300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate |
| Read speed | 2 minute (96-well plate) |
REAGENT DISPENSERS
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5-1000 µL in 1 µL increments |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 – 200 µL |
PHYSICAL CHARACTERISTICS
| Connectivity | 1 USB, 1 RS232 for external PC control |
| Power | 100-240 VAC, 50/60 Hz |
| Dimensions | 16″W x 15″D x 10″H (40.6 x 38 x 25.4 cm) |
| Weight | 40 lbs (18 kg) |
REGULATORY
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
| Part # | S1L | S1A | S1LA | S1LF | S1LFA | S1LFTA |
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Absorbance
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Fluorescence
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Luminescence
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Alpha
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Incubation
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Shaking
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Dual Reagent Injector*
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TRF (secondary mode)
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Software
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Gen5™ included with all configurations | |||||
*Dual Reagent Injector module is optional, must be purchased separately
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